Room disinfection by cold nebulization

Fast and effective room disinfection by cold nebulization


A special combination of active ingredients consisting of hydrogen peroxide and silver ions is atomized in the room using a special device. During this process, the hydrogen peroxide H2O2 decomposes without residue into its components water and oxygen – and thus disinfects surfaces and indoor air in a very friendly manner. Even areas that are difficult to access, which cannot be reached with wiping disinfection, are disinfected during cold nebulization (vaporization).


Cold nebulization does not replace traditional cleaning and ongoing disinfection, but offers effective additional disinfection – especially in challenging times such as the Corona/Covid-19 pandemic.


Use biocides safely. Always read the label and product information before use.

What are the advantages of disinfection with cold nebulization?


Works in all closed rooms and buildings. Disinfect thoroughly down to the last corner.

  • Disinfection of hard-to-reach areas and textile coverings
  • Disinfection of ventilation systems
  • Removal of unpleasant smells
  • Effective prevention in times of flu or Corona / Covid-19
  • Effective against bacteria, yeasts, skin fungi, molds, enveloped and uncovered viruses, mycobacteria and spores





Set up DISINFECT plus

Set up DISINFECT plus in the room. Make sure there is sufficient clearance. Check whether the device is level.


Prepare space

Close the window. Check whether there are still people or animals in the room.


Seal room

Seal ventilation openings airtight. Remove sensitive materials & equipment. Check the smoke detector and take any necessary measures.



Pay attention to protective equipment! Open the door on the front of the DISINFECT plus, open the canister lock and connect the hose to the canister. Place the canister with DPF (DISINFECT plus Fluid) on the scale so that the RFID tag is at the back.


Connect DISINFECT plus

Connect DISINFECT plus to a power source (230 V).


Protective clothing

Suitable protective equipment (eye and respiratory protection, gloves, protective clothing) must always be worn during the decontamination process!


Switch on DISINFECT plus

To switch on DISINFECT plus, press the on /off switch to the right of the touchscreen. To log in, either enter the pin code or hold the ID card to the card reader.


Start the program

Select the room and the desired program and start decontamination. DISINFECT plus starts with the countdown (adjustable, 5 min as standard). Start of decontamination!


Leave room

After starting the decontamination program, leave the room quickly and close the door behind you.


Seal the door

After leaving the room, seal the door with DISINFECT-Plus adhesive tape, lock the room and attach a safety sign to the door.



Only enter the room to change the canister during steaming. Pay attention to protective equipment!


Free up space

End of decontamination! Only release space for use when the hydrogen peroxide concentration is below the MAK value of 0.5 ppm - measure with a gas measuring device.



DISINFECT plus is a modern decontamination device that uses hydrogen peroxide (H2O2) to generate dry steam, thereby eliminating microorganisms and germs. The cold dry steam is a micro-aerosol and is generated by a patented ultrasound technology.

DCXF (DCX Fluid)


Decontamination with gaseous H2O2 is a highly effective decontamination process for rooms and areas with excellent compatibility for a variety of materials, electronic devices and any electrical systems. As a result, the preparation effort for decontamination is kept extremely low.


After decontamination, hydrogen peroxide decomposes to water and oxygen without leaving any residue. According to various hygiene research institutes, the use of “dry” fumigators with a low H2O2 concentration (between 4 and 8 percent) and a small amount of silver ions is very suitable for inactivating viruses, germs, bacteria, spores and fungi. DISINFECT plus uses a specially prepared 7.5% peroxide (DISINFECT plus fluid) for disinfection.


By adding special stabilizers, the medium can be stored for up to 18 months and is easy to store. Silver ions are also added to the liquid to support disinfection. The reagent is delivered in 10 liter canisters.



We offer special bio-indicators for monitoring decontamination. These indicators are extensively tested spores of Geobacillus stearothermophilus (ATCC # 12980), which is a particularly resistant but non-pathogenic germ. This way, the decontamination success of DISINFECT plus can be checked and confirmed safely and easily.

Product features:

The germs are fixed on stainless steel carriers.

A practical Tyvek / Tyvek® packaging with three Geobacillus stearothermophilus populations.

Three carriers are inoculated with Log 4, Log 5 and Log 6 spores, respectively, and sealed in separate compartments.

Thumb tab for peeling; perforated for any hanging.

Use of bio indicators:

The use and evaluation of the bioindicators are very easy. The bio-indicator packaging is placed unopened in the room to be decontaminated and gassed with DISINFECT plus. The DISINFECT plus bioindicator is then collected again, the three steel carriers are removed aseptically and each individual plate is placed in a labeled, sterile tube with sterile nutrient medium and incubated. An evaluation is carried out by means of an optical assessment of the tubes.


An essential feature of the DISINFECT plus bioindicators are the three different spore concentrations described above in a single, divided packaging. This enables a spatial distribution of the micro-aerosol to be investigated in relation to successfully decontaminated log stages. Since three different log levels (log 4, log 5 and log 6) can be gassed at one point using a single package, it is possible to determine exactly which reduction has been achieved for each position in the room.



A built-in industrial computer serves as a control system and database and is operated with the touchscreen monitor. Safety monitoring is provided by separate relay circuits. Two card readers are installed. One of them is used to log in via a login card, the second is used in combination with the DCXF canister (DCX Fluid) with RFID technology. Among other things, an electronic scale is used, which uses RFID technology to prevent tampering with the liquid and thus serves quality assurance.


Important process parameters such as temperature and humidity are continuously monitored and recorded. The decontamination process is controlled by the data obtained. All recorded data can be read out on the integrated USB interface.


The ultrasonic bath is monitored with level measuring devices. Here, min. and max. level continuously measured and checked. If the liquid level is too low, the pump is automatically activated. The built-in ultrasonic vibrators atomize the DCXF (DCX Fluid), which is located in the ultrasonic tank.


On the back, DISINFECT plus also has connection options for heating, dehumidifier, fan, ventilation and additional temperature and humidity sensors

Software control:

The software controls the most important hardware components by recording the operating times. The quality assurance of DISINFECT plus is made possible with RFID.


The documentation of the most important process parameters also serves for quality assurance and enables validation of the decontamination.

System monitoring:

Control of regulation instead of coordinated control – A certain amount of hydrogen peroxide is released into the room until a set humidity value is reached. After falling below a predetermined threshold, the process starts again. This control circuit enables an exact release of hydrogen peroxide into the room and also makes the results of the decontamination performance reproducible.


The ultrasonic transducers integrated in the DISINFECT plus generate the effective aerosol particles of the decontamination medium DISINFECT plus. These particles are filtered by the “acceleration prism” developed and patented by DISINFECT plus. This means that only the smallest parts, the microaerosols, with a droplet size of <1 µm can get into the environment.


In order to effectively distribute the microaerosol in the room, the aerosol is additionally accelerated by an accompanying, faster air flow and can therefore spread quickly.

RFID technology

DISINFECT plus is equipped with an RFID reader. The reagent liquid is in a 10 liter canister inside the DISINFECT plus. This canister has a built-in RFID chip that sends data about the liquid level and content to the system (batch monitoring via RFID).


The decontamination system is thus able to recognize the correct agent and its batch number, to check it and to protect it against unauthorized manipulation.


If the reagent level approaches the programmed lower limit, a warning is issued. If the minimum limit is not reached, the system is locked because it detects that there is insufficient liquid to completely decontaminate the room.

Technical device data



Dimensions mm (L / W / H) 1256 x 590 x 920
total weight approx. 90kg
Power consumption max. 600W
voltage 230V/50Hz
Relative humidity max. 90% occasionally up to 100%
room size 5m³ – 500m³
Communication USB, Network
Operating current (passive) 0,3A
Operating current (active) 2,5A
Degree of protection IP10
insulation class Class I + Class II arrangement
Process computer Intel X86, RAM 512kB, Windows XP or higher
Printer optionally via USB
Validable Yes

DCX H₂O₂ liquid


H₂O₂ concentration 7,5%
Addition silver ions and stabilizers
H₂O₂ canister volume 10 liters
Storage up to 30 ° C
Shelf life up to 18 months
Weight of canister (full) approx.10.25 kg

H₂O₂ gas generator


Humidity measurement 0 to 100% +/- 2% RH
Room operating temperature 15 to 30° C
Nebulization process Ultrasound
droplet size < 1 µm
Certificates /Test reports

Austrian Research Institute for Chemistry and Technology: “Checking the bactericidal, fungicidal and sporocidal effects of the DCX nebulizer system under practical conditions.”


MedUni Vienna, Medical-Technical Hygiene, Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectious Diseases and Immunology, Medical University of Vienna: “DCXF surface disinfection” without mechanics – bactericidal and fungicidal effects.”


Hygiene Nord GmbH, c/o BioTechnikum: “DCXF surface disinfection” without mechanics – bactericidal and fungicidal effect ”


Since the aerosol process is not yet subject to any standards, the disinfectant DCX-Fluid was recommended after thorough, successful tests for room disinfection:

ÖGHMP (Austrian Society for Hygiene, Microbiology and Preventive Medicine)

VAH (Association for Applied Hygiene)

Certified effectiveness of DCX:

The effectiveness has been tested by the private testing and research institute OFI (Austrian Research Institute for Chemistry and Technology) and tested and confirmed with suspension tests in the medical university of Vienna and in Hygiene Nord GmbH.


The tests were carried out in compliance with ÖNORM EN 13697 and the DGHM standard methods for testing chemical disinfection processes using various germ carriers.


By comparing the control and experimental groups, the excellent decontamination effect could be confirmed.

Test germs:

  • Staphylococcus aureus
  • Aspergillus niger
  • Geobacillus stearothermophilus
  • Clostridium difficile
  • Enterococcos hirae
  • Pseudomonas aeroginosa
  • Proteus mirabilis
  • Candida albicans
  • Escherichia coli
  • hNV( Humanes Norovirus)
  • FCV (Filine Calisivirus)